Innenrücktitelbild: Controlled Polymer‐Brush Growth from Microliter Volumes using Sacrificial‐Anode Atom‐Transfer Radical Polymerization (Angew. Chem. 35/2013)

Colorimetric quantitation of albumin in microliter volumes of serum.

Various non-immunochemical approaches to the quantitation of albumin in serum are reviewed. Salt fractionation techniques are unreliable, with substantial errors in estimating hypoalbuminemic states. Electrophoresis displays biases owing to irregular dye-binding or to densitometric scanning of irregular globulin bands. Currently, the most reliable colorimetric procedure for albumin quantitation...

Analysis of microliter volumes of dye-labeled nucleic acids.

We describe methodology useful for determining the fluorescent activity of small volumes (e.g., 1 microL) of dye-labeled cDNAs using microabsorbance spectroscopy. A direct relationship was found between the average fluorescent hybridization signal from an array and the fluorescent activity of the cDNA used in the hybridization. The microabsorbance method makes possible the screening of small-vo...

In vitro embryo culture in defined, sub-microliter volumes.

The high attrition rate of in vitro human embryo culture presents a major obstacle in the treatment of clinical infertility by in vitro fertilization (IVF). Physical and genetic requirements are not well understood for human or mouse preimplantation embryo development. Group culture is an established requirement for optimal embryo development in the mouse model. However, conventional microdrop ...

Block and Graft Copolymerization by Controlled/Living Radical Polymerization Methods

Noncontact temperature measurement in microliter-sized volumes using fluorescent-labeled DNA oligomers.

A new method of noncontact temperature measurement in microliter-sized volumes is demonstrated, based on the temperature sensitivity of the fluorescence lifetime of rhodamine-G when it is attached to a DNA oligomer. As temperature changes, the spacing between the fluorescent dye and a designed sequence of DNA bases is modulated by conformation changes of the DNA chain, and as a result the abili...